铜胁迫对小白菜叶肉细胞超微结构的影响
中国生态农业学报 2008 M7 »16 »4 ùChineseJournalofEco-Agriculture,July2008, 164948-951 DOI10.3724/SP.J.1011.2008.00948铜胁迫对小白菜叶肉细胞超微结构的影响**林义章1 张淑媛1 朱海生21.y j vÐó\Ðý Ü 350002;2.y 8 j, 、 å m1-6。“ Z ]”Ø i¹20 mg·L-1 H, s8 ì ˪ ´, ìË 58 ³n, ì Ø y, ¯ V4³ Ûs¿ s8Ï, s89 7 S 3MÄ,®ð ¥ è oMî om1-9; Ø i¹30mg·L-1 H, s8É Ëªø e w, ³8, û, _Ø R ì9m1-10;Ú} ç40 mg·L-1、50 mg·L-1 Ø/, s8ä ê, ì ´îÀ,Ç ¸ ˪m1-32, s8 、 ³8, s8¥Év7ᥠ_Ø R ì ¡,í ˪²m1-12, O s8X Ö 7C,CÉCs ÖCm1-23、m1-24。2.4 } çlâL ì8ѱ²¥Y} çlâL ì8¥Y l,VmÏ ¯ 4³“ô ¤” ½Ï¥L ì8 、,Ù ¹ b Vnm1 -28。Ú Ø/40mg·L-1、50 mg·L-1,“ô ¤”¥L ì8 så ,ö1VC¹Ùh ,ÉÏCÖ8²m1-29,L ì8 b Äm1-30。 Ø i¹20mg·L-1 H,“ Z ]” ½Ï¥L ì8 êîÁ ál o, C å m1-34; Ø i¹30mg·L-1 H,L ì8ÙM¤ y、 ´ bm1-35; Ø ir40mg·L-1 H,L ì8ÙÉ ,µ l oC,É s_ o|m1-36。3 Ö0} ç ²¥ åÊ, ±þ sý]¥ ], åÊñ | ¿±þ F¥ ,CÖ8², 30 U50mg·L-1 Ø¥L ì8C b Ä27~ 3050K;31“ Z ]”10 mg·L-1 Ø¥ ½, U s8 ì ˪ ¹ 60K;32“ Z ]”40mg·L-1 Ø¥ ½, U s8 ì ´îÀ,Ç ¸ ˪ph îU40K;33~ 36“ Z ]” ½Ï¥L ì8, 33 UvL ì8,Ù b , 34¹20 mg·L-1 Ø¥L ì8, êîÁ álo, 35 U30mg·L-1 Ø,L ì8Ù y、 ´ b, 36 U40mg·L-1 Ø¥L ì8É _ o|ph1 î U,iµ l oCph2 î U3340K;3450K;3560K;3680K。ChlChloroplast;CWCelwal;GraGranum;MMitochondrion;PPlasto-globulis.1~6ElectronmicrographsofWuyuemansmesophylcelsafectedby0mg·L-1, 10 mg·L-1, 20mg·L-1, 30mg·L-1, 40mg·L-1and50mg·L-1Custress, showthechangeofchloroplaststructure20K;7~12ElectronmicrographsofShanghaiqingsmesophyllcellsaf-fectedby0mg·L-1, 10mg·L-1, 20mg·L-1, 30mg·L-1, 40mg·L-1, 50mg·L-1Custress, showthechangeofchloroplaststructure20K;13~18ElectronmicrographsofWuyuemansmesophylcelsafectedby0mg·L-1, 10mg·L-1, 20mg·L-1, 30mg·L-1, 40mg·L-1, 50mg·L-1Custress, showthedecreasingcourseofchloroplastnumber6 000;19~24ElectronmicrographsofShanghaiqingsmesophylcelsafectedby0mg·L-1, 10mg·L-1,20mg·L-1, 30mg·L-1, 40mg·L-1, 50mg·L-1Custress, showthedecreasingcourseofchloroplastnumber3 000;950»4 ù lc©} çl⠽ѱ²¥Y25ElectronmicrographofthemagnificationofWuyuemansnormalmesophylcells, showsthegranumandstromalumen50K;26Electronmi-crographofWuyuemansmesophylcelafectedby30mg·L-1Custress, showsthecurvinggranumlumen, andvesicularsphereareobservedbetweenthecelwalandcytoplast50K;27~ 30ElectronmicrographsofWuyuemansmitochondria;27showsthenormalstructureofmitochondrion;28showsthestructureofmitochondrionafectedby10mg·L-1Custress;29showsthebreakdownofcristaeandappearanceofcertaincrystalstructureinmi-tochondrionwhenafectedby40mg·L-1 Custress;30 showsthevacuolizationofmitochondrionwhenafectedby50 mg·L-1 Custress27~ 3050K;31ElectronmicrographofShanghaiqingsmesophylcelsafectedby10mg·L-1 Custress, showstheorderlychloroplastgranumlumen60K;32ElectronmicrographofShanghaiqingsmesophylcelsafectedby40mg·L-1Custres, showsthegranumlumenbecomeingfloc, remainessomesegment40K;33~ 36ElectronmicrographsofShanghaiqingsmitochondria;33showsthenormalstructureofmitochondrion, 34 showsthestructureofmitochondrionafectedby20mg·L-1Custress, theectoblastcondensesintocompactglobule, 35showsthestructureofmitochondrionafect-edby30mg·L-1Custress, somecristaeofmitochondriaischaos, 36showsthestructureofmitochondrionafectedby40mg·L-1Custress, plasmale-mmawrinkleandsomevesicularsphereareobserved3340K;3450K;3560K;3680K.µ½ ç ; 3Á ï¥vl。µ¡·, s Íîh sE,ªªô Û“ s8²¥sÈ。 kV ü,® , s8VÈ¥õ ³nî o,Kª, 、 ï³。l⠽ѱ²} ç¥Qi ÖÕW¥µs,V L²T A,“ô ¤” ½¥ s8 、 Ú i} ç/³h ,7“ Z ]”5} ç i¹30mg·L-1 H s8 üC³8C, ª ü“ô ¤” “ Z ]” 0,V7¹lâF¥y ñÊ4 Ø G 。3.2 } çlâL ì8ѱ²¥YkV ü,L ì8 } ç/ ¹ 0 s¥。L ì8 Ë ºVñÏh ,µ ¦ ª¹Ð Ë¥« T¨µ1, Ë ºª ù, ¯³1L ì8« 4 MþÉ[10] 。Úi} ç/,“ô ¤” ½ÏsL ì8Ù³h ,C²Ö。Farrants©[11]YV²ÖÐ9 Ø£ùms/ £ L L ì8 Ö8d8¥i,i ª¹L ì8DNAy 3lM, VÁL ì8« ýÏ¥âÉVrsÈ,É7/îâÉ ½¥sÈ,VC¹Èä/ ÿòs¥Ë²Öd8。L ì8 ØÆ ÖÖÌ 3þÄÉ¥Ê,} ç/L ì8 ¥ åÊ、Ù¥h .Àh A|Y ØÆ ÖÖÌ 3þÄ¥É,V7Ylâ Ë¥ }。 IÓD[ 1] lc,· Å.ê Ú©±þ¥ 3 Øý]T¨ùî[J].ÏS 3 ÿ j¨l,¦¶ ,©.ê ؤ¼ ì 3É}Y¥ùî[J].¨ 3 ÿÐ, 2004, 151119-122[ 4] lc,· Å, 5.Cu} çlâ ¹ ½“d ðM1 F·S¥Y[J].y j vÐÐ,2007, 364369-372[ 5] õxâ.ír ¡ð ØÐ/ [M].ØÏS jñ, 199459[ 6] CheesemanJ.M.Mechanismofsalinitytoleranceinplants[J].PlantPhysiol., 1988, 87547-550[ 7] AsadaK.Thewater-watercycleinchloroplastsscaven-gingofactiveoxygensanddissipationofexcessphotons[J].Annu.Rev.PlantPhysiol.PlantMol.Biol., 1999,50601-639[ 8] ±Y, Ù¨, Iè,©. £ Ë¥ ºÐÑÄþ Ä ½ «VÄT¨¥1“[J].±þÐ,1984, 266605-615[ 9] JiangM.Y., YangW.Y., XuJ.Activeoxygendamageeffectofchlorophylldegrationinriceseedlingsunderos-moticstress[J].ActaBotanica, 1994, 364289-295[ 10] CrawfordS.A., WildensS.UltrastructuralaspectsofdamagetoleavesofEucalyptuscamaldulengisbythepsyl-lidcardiaspinarotator[J].Micron., 1996, 27359-366[ 11] FarrantsG.W., HovmollerS., StadhoudersA.M.Twotypesofmitochondrialcrystalsindiseasedhumanskeletalmusclefibers[J].MuscleNerve., 1988, 1145-55951