铜胁迫对小白菜叶肉细胞超微结构的影响
p中国生态农业学报 2008 M7 16 4 ChineseJournalofEco-Agriculture,July2008, 164948-951 nbsp; DOI10.3724/SP.J.1011.2008.00948铜胁迫对小白菜叶肉细胞超微结构的影响**林义章1 张淑媛1 朱海生21.y j v\ 350002;2.y 8 j, 、 m1-6。“ Z ]” i20 mgL-1 H, s8 , 58 n, y, V4 s s8, s89 7 S 3M, oM om1-9; i30mgL-1 H, s8 e w, 8, , _ R 9m1-10;} 40 mgL-1、50 mgL-1 /, s8 , , m1-32, s8 、 8, s8v7 _ R nbsp;, m1-12, O s8X 7C,CCs Cm1-23、m1-24。2.4 } lL 8Y} lL 8Y l,Vm 4“ ” L 8 、, b nbsp;Vnm1 -28。 /40mgL-1、50 mgL-1,“ ”L 8 s ,1VCh gt;,C8m1-29,L 8 b m1-30。 i20mgL-1 H,“ Z ]” L 8 l o, C m1-34; i30mgL-1 H,L 8M y、 bm1-35; ir40mgL-1 H,L 8 , l oC, s_ o|m1-36。3 0} , s] ], | F ,C8, 30 U50mgL-1 L 8C b 27~ 3050K;31“ Z ]”10 mgL-1 , U s8 nbsp;60K;32“ Z ]”40mgL-1 , U s8 , ph U40K;33~ 36“ Z ]” L 8, 33 UvL 8, b , 3420 mgL-1 L 8, lo, 35 U30mgL-1 ,L 8 y、 b, 36 U40mgL-1 L 8 _ o|ph1 U,i l oCph2 U3340K;3450K;3560K;3680K。ChlChloroplast;CWCelwal;GraGranum;MMitochondrion;PPlasto-globulis.1~6ElectronmicrographsofWuyueman39;smesophylcelsafectedby0mgL-1, 10 mgL-1, 20mgL-1, 30mgL-1, 40mgL-1and50mgL-1Custress, showthechangeofchloroplaststructure20K;7~12ElectronmicrographsofShanghaiqingsmesophyllcellsaf-fectedby0mgL-1, 10mgL-1, 20mgL-1, 30mgL-1, 40mgL-1, 50mgL-1Custress, showthechangeofchloroplaststructure20K;13~18ElectronmicrographsofWuyueman39;smesophylcelsafectedby0mgL-1, 10mgL-1, 20mgL-1, 30mgL-1, 40mgL-1, 50mgL-1Custress, showthedecreasingcourseofchloroplastnumber6 000;19~24ElectronmicrographsofShanghaiqing39;smesophylcelsafectedby0mgL-1, 10mgL-1,20mgL-1, 30mgL-1, 40mgL-1, 50mgL-1Custress, showthedecreasingcourseofchloroplastnumber3 000;9504 lc} l Y25ElectronmicrographofthemagnificationofWuyueman39;snormalmesophylcells, showsthegranumandstromalumen50K;26Electronmi-crographofWuyueman39;smesophylcelafectedby30mgL-1Custress, showsthecurvinggranumlumen, andvesicularsphereareobservedbetweenthecelwalandcytoplast50K;27~ 30ElectronmicrographsofWuyueman39;smitochondria;27showsthenormalstructureofmitochondrion;28showsthestructureofmitochondrionafectedby10mgL-1Custress;29showsthebreakdownofcristaeandappearanceofcertaincrystalstructureinmi-tochondrionwhenafectedby40mgL-1 Custress;30 showsthevacuolizationofmitochondrionwhenafectedby50 mgL-1 Custress27~ 3050K;31ElectronmicrographofShanghaiqing39;smesophylcelsafectedby10mgL-1 Custress, showstheorderlychloroplastgranumlumen60K;32ElectronmicrographofShanghaiqing39;smesophylcelsafectedby40mgL-1Custres, showsthegranumlumenbecomeingfloc, remainessomesegment40K;33~ 36ElectronmicrographsofShanghaiqing39;smitochondria;33showsthenormalstructureofmitochondrion, 34 showsthestructureofmitochondrionafectedby20mgL-1Custress, theectoblastcondensesintocompactglobule, 35showsthestructureofmitochondrionafect-edby30mgL-1Custress, somecristaeofmitochondriaischaos, 36showsthestructureofmitochondrionafectedby40mgL-1Custress, plasmale-mmawrinkleandsomevesicularsphereareobserved3340K;3450K;3560K;3680K. ; 3 vl。, s h sE, “ s8s。 kV , , s8V n o,K, 、 。l } Qi Ws,V39; LT A,“ ” s8 、 i} /h gt;,7“ Z ]”5} i30mgL-1 H s8 C8C, “ ” “ Z ]” 0,V7lFy 4 G nbsp;。3.2 } lL 8YkV ,L 8 } / 0 s。L 8 Vh gt; , T1, , 1L 8 4 M[10] 。i} /,“ ” sL 8h gt;,C。Farrants[11]YV9 ms/ L L 8 8d8i,i L 8DNAy 3lM, VL 8 nbsp;Vrs,7/ s,VC/ sd8。L 8 3,} /L 8 、h .h gt;A|Y 3,V7Yl }。 ID[ 1] lc, . 3 ]T[J].S 3 jl, ,. 3}Y[J]. 3 , 2004, 151119-122[ 4] lc, , 5.Cu} l “d M1 FSY[J].y j v,2007, 364369-372[ 5] x.r / [M].S jlt;, 199459[ 6] CheesemanJ.M.Mechanismofsalinitytoleranceinplants[J].PlantPhysiol., 1988, 87547-550[ 7] AsadaK.Thewater-watercycleinchloroplastsscaven-gingofactiveoxygensanddissipationofexcessphotons[J].Annu.Rev.PlantPhysiol.PlantMol.Biol., 1999,50601-639[ 8] Y, , I,. VT1“[J].,1984, 266605-615[ 9] JiangM.Y., YangW.Y., XuJ.Activeoxygendamageeffectofchlorophylldegrationinriceseedlingsunderos-moticstress[J].ActaBotanica, 1994, 364289-295[ 10] CrawfordS.A., WildensS.UltrastructuralaspectsofdamagetoleavesofEucalyptuscamaldulengisbythepsyl-lidcardiaspinarotator[J].Micron., 1996, 27359-366[ 11] FarrantsG.W., HovmollerS., StadhoudersA.M.Twotypesofmitochondrialcrystalsindiseasedhumanskeletalmusclefibers[J].MuscleNerve., 1988, 1145-55951/p