低浓度NaHSO3处理对温室黄瓜光合作用的影响
·ùî·® iNaHSO3 ØÑ iÛ;T¨¥Y*¢mu1, 2, Ù Wô2, Î3,¦ 41.安徽农业大学园艺学院, 安徽合肥 230036;2.上海市农业科学院, 上海市设施园艺技术重点实验室,上海 201106;3.上海交通大学农业与生物学院, 上海 201101;4.上海市宝山区生物技术中心, 上海 201907K 1¹® ið ; Ö÷¿Û;T¨¥Y Å,Ñ i ¡Hq/,[Û ÖÕDeltastar¹ k,¨® i¥NaHSO3 ·A ¨ Ø] 3â ù¥Û Ë。²TV ü® iNaHSO3 V[4ÚÑiÛ Ë¥; Î q。Ñ iÛNaHSO3 Ø¥ aR i¹2.0 mmol/LP·,Û ì ù 7½² ù ¨® iNaHSO3 ·A V4ÚÛ Ë¥; Î q,i ûÃT¨3 dP·。Ðv ¨ £M1,2.0 mmol/LNaHSO3 Ø4ÚÛÿ Ë; Î q20 P·, OÐX©¥\ÉÖÌ;ÖÄ¥£ùy0PMS¥T¨ Ë », 9F ATP¥,7idY Ë s ÍPSⅡ¥;ÄÐr q。ÓcÎ ;vf /NaHSO3¥¨É 。1oMÛ;Ñ i;;T¨;NaHSO3Ïms Ë|Q945.11ÓDS M AÓcI|1007-7146200903-0295-05EffectsofLowConcentrationNaHSO3 TreatmentonPhotosynthesisofCucumberinGreenhousePEIXiao-bo1, 2, LIShi-cheng2, CAIRun3, WANGYue41.SchoolofHorticulture, AnhuiAgriculturalUniversity, Hefei230036, Anhui, China;2.ShanghaiAcademyofAgriculturalScience, ShanghaiKeyLaboratoryofProtectedHorticulturalTechnology, Shanghai201106, China;3.CollegeofAgronomyandBiology, ShanghaiJiaotongUniversity, Shanghai201101, China;4.CenterofBiotechnologyofBao-shan, Shanghai201907, ChinaAbstractToprobetheinfluenceandmechanismoflowconcentrationofsulfiteonphotosynthesisofcucumber, onecu-cumbervariety“Deltastar” waschosenandsprayedwithNaHSO3 atleavesinlowconcentrationatdiferentstages, theresultsindicatedthatthephotosynthesisrateofleaveswasimprovedingreenhouse.Theoptimalconcentrationofcucum-berwasabout2.0 mmol/L.Thetreatmentwaseffectivewhencucumberatplantletoratblooming fruitingstage, withcontinuouslyeffectmaintainedforabout3 days.Comparedtothecontrolsprayingwater, thephotosynthesisrateofcucumberfunctionalleaveswasimprovedby20 with2.0 mmol/LNaHSO3 treatment, inaccordwithPhenazinemet-hosulfatePMSwhichisacofactorofcatalyzingcyclic-photophosphroylation, raisingtheATPcontentintheleaves.AndthepotentialphotochemicalefficiencyofPSⅡ wasnotafectedbysprayinglowconcentrationofNaHSO3.Applica-tionofNaHSO3 intoprotectedcultureunderinsuficientlightconditionwasalsodiscussedinthearticle.»18 »3 ù2009 M6 ; 3 þ Ð ACTA LASER BIOLOGY SINICA Vol.18 No.3Jun.2009* là ° ù2008-09-15;©í ° ù2009-01-10Á[ “SE S/¦ê9Ã[ “2008BADCOB02;SE1 SÐÁÄ[ “39830230;½ê 81 SÐÁ[ “03041106;½ê 8ª® ¦ ¡[ “Teº1970, 3,½êÈ a ¦,¬ q,p V,ö1ùîZ_¹ ó\。È0¯Q;Keywordscucumber;greenhouse;photosynthesis;Sodiumbi-sulfiteNaHSO3Tþ ¨ ÒNaHSO3[4Ú ;T¨9FÁ ¥÷EáSXÜ k¨[ 1-3] ,µ¬î¹BñN÷、、í]、µr7 j 3ÁÏ Vw¨¥zn 。* ù¥ùî ª¹ 4Ú;T¨¿NaH-SO3]Å ±þ;« ¥ø,i| T¹B Ë;« ]Å4 P¨。[/ ¥ Ø ùîVü[ 4-9] ,® iNaHSO3;T¨¥\Ér¿ñYV\ɶ ¡PSI¥ÖÌÈ0.¾ Ò ó¥;ÖÄ,9FATP¥,i ®¿ ñ]Å ;« ,\É d 7,T¹; 2¥ 3Éâ[ îA³¥ ;g7 T¨¥。;T¨ ±þK1¥ 3 ØVñ,9 ±þîÄþÉÐÁ ¥。]Tþ NaHSO3Ø i rTXµ[ 10-12] , ÂÇÍ ¨5 mol/LNaHSO3 h ù®Ñ;;T¨¥Y, 100 mg/LNaHSO3µr¹4Ú ö Ë ÑË; ä Í¥c , m 3¯ ¨200 mg/LNaH-SO3 4Ú; Î q s Íc , P¯9Á4Ú ÖÉ。2 mmol/LNaHSO3 V Pl . ìÁ 9F, P b ÖÎð ïh Ð,KÔÁ. ìâÉc/。YVNaHSO3 ØÑ iÛ¥;T¨Y Å¥,Y ;v f /NaHSO3 Ø \ÉÑ iÛ¥; Î q, ¹ 3Á ¥¨4G 。LÏ,¨É ¡A9à 1î“d eÅ, £ LVñÏÛ±é 3âBÁ。 æ¨Ñ i { ,Ç LC kÏ Ð;Ì⥠eÅBÁç,7 O VC L 3ÁÏ¥;v¥ f。1 ÐZEL ÖÕ¹ÛCucumissativusL., §Ñ i¨ ÖÕzDeltastar, RUKZWAAN ³。 L¿2005 M Z j SÐýj ðÑ iÏÉ。L¨ èû ¡,È5 Ø。 Û uF 9, Äl u30é, 3Q¯。NaHSO3 ØsY¿Û ìù、 7½² ù±é ¥ Ë ¨ ÒNaHSO3。¿ dÊ4É ]、ÊBÁ¥î ˨ѱ ¨üÉ ¨ü Ø,[ ¨ © Ö0 £¹vCK。;T¨¥; Y wL©ç¨Li-6400;N。NaHSO3 ØªÛ Ë; Î q¥BW©ç¨Li-6400;N©ç。ATPc ¥©çvÓD[ 13]¥ZE,Ê | §ë¥Ûî ÿ ,5|Ï “,[Ï §¥ösYTv ¨ Ö0 £Ð Ø ¨2 mmol/LNaHSO3,¿Q °g | M]Ê、 ëM©¥ Ë ,m £ Ø ê µi4 |10 min, Ï ª, |Bç8 ·A¨; Í ½E©ç Ï¥ATPc 。 s Í; ¥©ç¨PAM-2100Lz TØÅ;NWalz, Efeltrich, Germany。 æ¨ ¥±; 0/Ñh¥C2030-BCþ Ë,¿M]Ê Ë©ç s Í; 。© çÂ/ÜV s20 minÀ a¥ Ë,¨B l¿0.1 μmol/m2·s¥© ; Ô q¹600 Hzv ,¤ S;F0;u 7Bñ ;8 000 μmol/m2·s, 20 KHz, 0.8 s,¤À a/¥Kv;Fm;PSI¥Kv;ÄÐr qFv/Fm¨/ T9 ØFv/FmFm-F0/Fm。 Û©ç6é,¯3Q。2 ²TÐs2.1 NaHSO3 ØÑ iÛ Ë; Î q¥Y¨] iNaHSO3 ¨ ÒÑ iÛ±é Ë,Q° ö1 Ë¥; Î qm1, TÐl ¥ùî²TM », Ø Ë¥; Î qPn üAÚ¿v,Ñ iÛ NaHSO3¥ aR i¹2 mmol/LP·。1 2 mmol/LNaHSO3 Øv Ë¥;T¨;Y wLm2, VnNaHSO3ت, Ë;/ Ð;/¥; Î q ¤\É。vBHq/sY¿Ñ iÛ ì ù 7½² ùÛ ËÉ2 mmol/LNaHSO3 Ø,]¥; Hq/m3 Vµr4Ú; Î q20 [ ,N\ÉT¨ VûÃ3 dP·m4。2.2 NaHSO3PMS ØÛ Ë; Î q¥Y±þ ËÏy;ÖÄ4ATP ¡]ij17î¹;T¨¥KÅy0,|bÄÖÌ;ÖÄ¥£ùy0 ; ÖJqPMSÆ ËÈ V9F ËÏATPc 4Ú;r q。296 ; 3 þ Ð »18 Û 7½ ù,¨2.0 mmol/LNaHSO3 Ø¥Û Ë,»21000©ç¥ATPc μmol/m2¹5.12 ±0.37,7vϺµ3.01 ±0.23P0.033,µsA÷。1 2.0 mmol/LNaHSO30.2μmol/LPMSsY Ø ] ØÛ Ë;Î q¥Ym5,²TV ü, ñ ÌsY Ø ] Ø V\É; Î q20 P·,sY ØÐ ] Ø î4Ú; Î q¥ñM »,V ü ñ Ì¥\ÉT¨ V 9F ATP¥î,y7 ] ØÃVCßFr。|bÄÖÌ;ÖÄ¥£ùy0 ; ÖJqPMS Æ Ë V9F ËÏATPc 4Ú;r q。297»3 ù ¢mu©® iNaHSO3 ØÑ iÛ;T¨¥Y NaHSO3处理浓度为 2.0 mmol/L, PMS浓度为 0.2μmol/L,测定光强为 1 000 μmol/m2.s。Treatedwith2 mmol/LNaHSO3 and/orconcentrationofPMS0.2 μmol/L, measuredwithlightintensityof1 000 μmolphotons/m·s.图 5 花期NaHSO3和PMS处理对黄瓜叶片净光合速率的影响Fig.5 TheeffectofNaHSO3 andPMSonnetphotosynthsisrateincucumberleafatbloomstage2.3 NaHSO3 ØÛ Ë s Í;¥Y s Í; Fv/FmT¹ PSⅡ¥1; , ñQPSⅡ¥;ÄÐr q。 Âm6 îU, 0.5 mmol/L~ 4.0 mmol/LNaHSO3 ØÑ iÛ s ÍFv/FmYA÷,V üPSⅡid®iNaHSO3 ØY¥ÊÄ。CK, A, B, C, D, E分别表示对照, 0.5 mmol/L, 1.0mmol/L, 2.0 mmol/L, 3.0 mmol/L和 4.0 mmol/LNaHSO3处理。CK, A, B, C, DandErepresentcontrol, andtreatedwithNaHSO3 atconcentrationmmol/Lof0.5, 1.0, 2.0.3.0and4.0 respectively.图 6 黄瓜花期 NaHSO3处理对 PSⅡ光化学效率Fv/Fm的影响Fig.6 TheefectofNaHSO3 onthepotentialphotochemi-caleficiencyFv/FmofPSⅡ incucumberatbloomstage3 Tþ ¨ ® iNaHSO3\É; Î q¥T¨, Ö8 L8 LÏ ¤[£ L[ 4-6] ,NaH-SO34Ú; Î qô Û“ ñ\É Ë ¥;ÖÄ,9F s8îATP¥ ï,ö1By¿ ñ\É ¶ ¡PSⅠ¥Ì TÈ0.¾[ 9] 。 - ¦ùî ª¹[ 14-15] ,±þ ËÏy;ÖÄ4ATP ¡]ij17î¹;T¨¥KÅy0,|bÄÖÌ;ÖÄ¥£ùy0 ; ÖJqPMS Æ ËÈ V9F ËÏATPc 4Ú;r q。 LϨNaHSO3X© V\ÉÖÌ;ÖÄ¥£ùy0PMS ] ØÛ Ë¥;Î q4ÚíßFr,£ L NaHSO3 Ø Ë4Ú; Î q¥ðyö1 \É ;ÖÄ9FATP¥ø。¿ 2Z 3Á7ý,Y C}Ñ i,ë1«Ñ i K®ÑBî»û20 ℃P·,ÜÈC;v õ|Ï ,;v Û¥ÄÁ v/,ªªî¹ Kö1¥KÅy0[ 16] 。V L¥²T A,Ñ i 3ÁÏí Ûì ùÎ 7½² ù, Ë ¨ Ò2 mmol/LNaHSO3µrT, V4Ú ; Î q20 P·;Ñ i ¥;vPAR Ú®,® iNaHSO3 Ø µrT, OPAR®¿200 μmol/m2·s H,NaHSO3 Ø4Ú Ë; Î q¥rT÷F üAm3、m4。yN, 3ÁÏ ¨ Ò® iNaHSO3Ç 4Ú Ë; Î q,7 O¹Ï³h ;v 3Á{ ¥µ ëY4 BHoå。References[ 1] ¸ÖP,B Y.ð ; Ö _ N0 3Éâ¥Y[ J].jÐ/ , 1993, 430-32.XUYu-xiang, TIANShu-fang.EfectofNaHSO3 onGrowthandDevelopmentofEggplant[ J].AgronomyandTechnology,1993, 430-32.[ 2]¦ld,ñkê.ð ; Ö _ ö Ë Ñ ¥¨ùî[J].T SÐ, 1991, 8297-99.WANGYi-zhang, WUXiao-dong.AppliedResearchofSodiumBisulfiteonDangshanPear[ J].FruitScience, 1991, 8297-99.[ 3]p. , O.ð ; Ö _ m 3¯Á Ð ÖÉ¥¿ ÿ[J].ÏS jÐY, 2000, 16415-17.TUChuan-lin, KETan.ImprovementofTeaYieldandQualitybyNaHSO3 inHilArea[J].ChineseAgriculturalScienceBulle-tin, 2000, 16415-17.[ 4]L, Ñ.ð ; Ö _ ; ¥Y[J].±þ 3 ØÐ, 1987, 1342-50.298 ; 3 þ Ð »18 TANShi, SHENYun-gang.TheEfectofSodiumBisulfiteonPhotosyntheticApparatusandItsOperation[J].Phytophysiologi-ca, 1987, 1342-50.[ 5]ÉE å, Ñ, Ù£2,©.ð ; 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