低浓度NaHSO3处理对温室黄瓜光合作用的影响
p iNaHSO3 i;TY*mu1, 2, W2, nbsp;3, 41.安徽农业大学园艺学院, 安徽合肥 230036;2.上海市农业科学院, 上海市设施园艺技术重点实验室,上海 201106;3.上海交通大学农业与生物学院, 上海 201101;4.上海市宝山区生物技术中心, 上海 201907K 1 i ; ;TY , i Hq/,[ Deltastar k, iNaHSO3 A ] 3 。TV iNaHSO3 V[4i ; q。 iNaHSO3 aR i2.0 mmol/LP, 7 iNaHSO3 A V4 ; q,i T3 dP。v M1,2.0 mmol/LNaHSO3 4 ; q20 P, OX\;y0PMST , 9F ATP,7idY s PSⅡ;r q。c ;vf /NaHSO3 。1oM; i;;T;NaHSO3ms |Q945.11DS M AcI|1007-7146200903-0295-05EffectsofLowConcentrationNaHSO3 TreatmentonPhotosynthesisofCucumberinGreenhousePEIXiao-bo1, 2, LIShi-cheng2, CAIRun3, WANGYue41.SchoolofHorticulture, AnhuiAgriculturalUniversity, Hefei230036, Anhui, China;2.ShanghaiAcademyofAgriculturalScience, ShanghaiKeyLaboratoryofProtectedHorticulturalTechnology, Shanghai201106, China;3.CollegeofAgronomyandBiology, ShanghaiJiaotongUniversity, Shanghai201101, China;4.CenterofBiotechnologyofBao-shan, Shanghai201907, ChinaAbstractToprobetheinfluenceandmechanismoflowconcentrationofsulfiteonphotosynthesisofcucumber, onecu-cumbervariety“Deltastar” waschosenandsprayedwithNaHSO3 atleavesinlowconcentrationatdiferentstages, theresultsindicatedthatthephotosynthesisrateofleaveswasimprovedingreenhouse.Theoptimalconcentrationofcucum-berwasabout2.0 mmol/L.Thetreatmentwaseffectivewhencucumberatplantletoratblooming fruitingstage, withcontinuouslyeffectmaintainedforabout3 days.Comparedtothecontrolsprayingwater, thephotosynthesisrateofcucumberfunctionalleaveswasimprovedby20 with2.0 mmol/LNaHSO3 treatment, inaccordwithPhenazinemet-hosulfatePMSwhichisacofactorofcatalyzingcyclic-photophosphroylation, raisingtheATPcontentintheleaves.AndthepotentialphotochemicalefficiencyofPSⅡ wasnotafectedbysprayinglowconcentrationofNaHSO3.Applica-tionofNaHSO3 intoprotectedcultureunderinsuficientlightconditionwasalsodiscussedinthearticle.18 3 2009 M6 nbsp; nbsp; nbsp; nbsp; nbsp; ; nbsp;3 ACTA LASER BIOLOGY SINICA nbsp; nbsp; nbsp; nbsp; nbsp; Vol.18 No.3Jun.2009* l 2008-09-15; 2009-01-10[ “SE S/9[ “2008BADCOB02;SE1 S[ “39830230; 81 S[ “03041106; 8 [ “Te1970, 3, a , q,p V,1Z_ \。0Q;Keywordscucumber;greenhouse;photosynthesis;Sodiumbi-sulfiteNaHSO3T NaHSO3[4 ;T9F ESX k[ 1-3] ,BN、、]、r7 jlt; 3 Vwlt;zn 。* 4;TNaH-SO3] ; ,i| TB ; ]4 P。[/ V[ 4-9] , iNaHSO3;T\rYV\ PSI0. ;,9FATP,i ] ; ,\ d 7,T; 2 3[ A ;g7 T。;T K1 3 V,9 。]T NaHSO3 i rTX[ 10-12] , 5 mol/LNaHSO3 h nbsp;;;TY, 100 mg/LNaHSO3r4 ; c , m 3 200 mg/LNaH-SO3 4; q s c , P94 。2 mmol/LNaHSO3 V Pl . 9F, P b h ,K. c/。YVNaHSO3 i;TY ,Y ;v f /NaHSO3 \ i; q, 3 4G nbsp;。L, A9 1“d e, LV 3B。 i { , LC k ; eB,7 O VC L 3;v f。1 ZEL CucumissativusL., i zDeltastar, RUKZWAAN 。 L2005 M Z jlt; Sj i。L ,5 。 uF 9, l u30, 3Q。NaHSO3 sY 、 7 NaHSO3。 d4 ]、B ,[ 0 vCK。;T; lt;Y wLLi-6400;N。NaHSO3 ; qBWLi-6400;N。ATPc vD[ 13]ZE, | ,5| “,[ sYTv 0 2 mmol/LNaHSO3,Q g | M]、 M ,m i4 |10 min, , |B8 A; E ATPc 。 s ; PAM-2100Lz T;NWalz, Efeltrich, Germany。 ; 0/hC2030-BC ,M] s ; 。 /V s20 min a ,B l0.1 μmol/m2s ; q600 Hzv , S;F0;u 7B ;8 000 μmol/m2s, 20 KHz, 0.8 s, a/Kv;Fm;PSIKv;r qFv/Fm/ T9 Fv/FmFm-F0/Fm。 6,3Q。2 Ts2.1 NaHSO3 i ; qY] iNaHSO3 i ,Q 1 ; qm1, Tl TM , ; qPn Av, i NaHSO3 aR i2 mmol/LP。1 2 mmol/LNaHSO3 v ;T;Y wLm2, VnNaHSO3, ;/ ;/; q \。vBHq/sY i 7 2 mmol/LNaHSO3 ,]; lt;Hq/m3 Vr4; q20 [ ,N\T V3 dPm4。2.2 NaHSO3PMS ; qY y;4ATP ]17;TKy0,|b;y0 ; JqPMS V9F ATPc 4;r q。296 nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; ; nbsp;3 nbsp; nbsp; nbsp; nbsp; nbsp; 18 7 ,2.0 mmol/LNaHSO3 ,21000ATPc μmol/m25.12 0.37,7v3.01 0.23P0.033,sA。1 2.0 mmol/LNaHSO30.2μmol/LPMSsY ] ; qYm5,TV , sY ] V\; q20 P,sY ] 4; qM ,V \T V 9F ATP,y7 ] VCFr。|b;y0 ; JqPMS V9F ATPc 4;r q。2973 nbsp; nbsp; nbsp; nbsp; nbsp; mu iNaHSO3 i;TY nbsp; nbsp; nbsp; nbsp; nbsp;NaHSO3处理浓度为 2.0 mmol/L, PMS浓度为 0.2μmol/L,测定光强为 1 000 μmol/m2.s。Treatedwith2 mmol/LNaHSO3 and/orconcentrationofPMS0.2 μmol/L, measuredwithlightintensityof1 000 μmolphotons/ms.图 5 花期NaHSO3和PMS处理对黄瓜叶片净光合速率的影响Fig.5 TheeffectofNaHSO3 andPMSonnetphotosynthsisrateincucumberleafatbloomstage2.3 NaHSO3 s ;Y s ; Fv/FmT PSⅡ1; , QPSⅡ;r q。 m6 U, 0.5 mmol/L~ 4.0 mmol/LNaHSO3 i s Fv/FmYA,V PSⅡidiNaHSO3 Y。CK, A, B, C, D, E分别表示对照, 0.5 mmol/L, 1.0mmol/L, 2.0 mmol/L, 3.0 mmol/L和 4.0 mmol/LNaHSO3处理。CK, A, B, C, DandErepresentcontrol, andtreatedwithNaHSO3 atconcentrationmmol/Lof0.5, 1.0, 2.0.3.0and4.0 respectively.图 6 黄瓜花期 NaHSO3处理对 PSⅡ光化学效率Fv/Fm的影响Fig.6 TheefectofNaHSO3 onthepotentialphotochemi-caleficiencyFv/FmofPSⅡ incucumberatbloomstage3 T iNaHSO3\; qT, 8 L8 L [ L[ 4-6] ,NaH-SO34; q “ \ ;,9F s8ATP ,1By \ PSⅠ T0.[ 9] 。 - [ 14-15] , y;4ATP ]17;TKy0,|b;y0 ; JqPMS V9F ATPc 4;r q。 LNaHSO3X V\;y0PMS ] ; q4Fr, L NaHSO3 4; qy1 \ ;9FATP。 2Z 37,Y C} i,1 i KB20 ℃P,C;v | ,;v v/, K1Ky0[ 16] 。V39; LT A, i 3 7 , 2 mmol/LNaHSO3rT, V4 ; q20 P; i ;vPAR , iNaHSO3 rT, OPAR200 μmol/m2s H,NaHSO3 4 ; qrTF Am3、m4。yN, 3 iNaHSO3 4 ; q,7 Oh ;v 3{ Y4 BHo。References[ 1] P,B Y. ; _ N0 3Y[ J].jlt;/ , 1993, 430-32.XUYu-xiang, TIANShu-fang.EfectofNaHSO3 onGrowthandDevelopmentofEggplant[ J].AgronomyandTechnology,1993, 430-32.[ 2]ld,k. ; 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