海水胁迫对菠菜(Spinacia olerancea L.)叶绿体活性氧和叶绿素代谢的影响
»29 »8 ù2009 M83 ÿÐACTAECOLOGICASINICAVol.29,No.8Aug., 2009htp//Á[ “SEÚnp VÄ SùÁ ù[ “20050307031; Ê 81 SÐÁ ù[ “BK2006140là ° ù2008-05-09; ©è ° ù2008-12-10*YßTCorrespondingauthor.E-Z £} çkSpinaciaoleranceaL. s8 s Í}¥Y孙 锦1, 2,贾永霞1,郭世荣1,*,李 娟11. 2Ø jvÐó\Ðý, 2Ø 210095;2.Å Õ 8 j SÐý vùî î, §Ü 730070K1[Z £ ùË ÖÕõk 0Z £ ÖÕ §3| ¹ k,¨ £ ¡ZE,ùî Z £} çkSpinaciaoleranceaL. s8ROS s ÍChl}¥Y。²TV ü,Z £} ç/, 2ñk ÖÕ s8 Ñ| Ö0O-·2 Á 3 Îq、VÄ _H2O2d MDAc A÷ 6Ú,i Oõk¥4Úv¿ §3| ;õk s8 ¥ÑÄþ Ä ½SOD、 FÊÓ ÖVÄþ ½APX、ÅtÎð ½GR FÊÓ ÖAsA、ÎðÅtGSHc ®¿ §3| ,7VÄþ ½PODÚ¿ §3| ;;Ä4J,ÚMVÉB P2ñk ÖÕ s8 O-·2Á 3Î qF y、H2O2c 4Ú、 ÉVÄF, b“4AsA üA® k s8 ROS £ Ü,ϳ ®MV/î¥ø¥ ÉVÄ ]。Z £} ç/, 2ñk ÖÕ Ë s ÍbChlb、 s ÍaChla î -8þÉð s Í ÖPchl、 ÃðúⅨMg-protoIX、ðúⅨProtoⅨ UúðⅢUroⅢc üA®,7 ä ÍðPBGδ-¼[û ÖALAÆ,Chlî sE¸,i Oõk¥ sEñv¿ §3| ;MVÉBF Õ sEñ,7AsAsϳ ®Z £} çMV/î¥E¸T¨。Z £} ç üA4Ú õk Ë s Í ½Chlase7 §3| ÀµY,MV ØõkChlase¥Yñv¿ §3| ,AsA2ñ ÖÕ ËChlase Àµ üAY。 ²T ª ü,Z £}ç/,k s8 ROSÐChl} á MM1,ÇYV s8 ¥Ä ] PChl³,7 O PChlî¥PBG_UroⅢÄç sE。 0Z £ ÖÕ §3| s8 b“ROSö1YVSODAsA-GSHÖÌ“d, b“ ï ,h ROS s8 ¥Ä å Chlî¥ sEñ,i OZ £} ç Chlase¥Y l;7Z £ ùË ÖÕõk s8 b“ROSö1G ¿SODPOD,ROS¥ b“ ïµK,V7Á ROSv Æ, s8 ¥Ä å Chlî¥ sEñø,i OZ £} çA÷4Ú Chlase,F Chl³。1oMZ £;kSpinaciaoleranceaL.; s8;; s ÍÓcI|1000-0933200908-4361-11 Ïms Ë|Q143, Q945, Q948 ÓDS M AEffectsofseawaterstressonmetabolismofreactiveoxygenspeciesandchlorophylinchloroplastsofspinachSpinaciaoleranceaL.SUNJin1, 2, JIAYong-Xia1, GUOShi-Rong1,*, LIJuan11ColegeofHorticulture, NanjingAgriculturalUniversity, Nanjing210095, China2VegetableInstitute, GansuAcademyofAgriculturalSciences, Lanzhou730070, ChinaActaEcologicaSinica, 2009, 2984361~ 4371.AbstractTheefectsofseawaterstresonmetabolismofreactiveoxygenspeciesROSandchlorophylChlinchloroplastoftwospinachcultivars, YuanyespinachseawatersensitivecultivarandHelanNo.3 seawatertolerantcultivarwereinvestigatedbyhydroponics.TheresultsshowedthatproductionrateofsuperoxideradicalO-·2, contentofhydrogenperoxideH2O2andmalonaldehydeMDAinchloroplastoftwospinachcultivarswereremarkablyincreasedbyseawaterwithhigherrateincv.Yuanye;Underthestresofseawater, theactivityofanti-oxidationenzymesuchassuperoxidedismutaseSOD, ascorbicacidperoxidaseAPXandglutathionereductaseGR, andthecontentofhtp//antioxidantsuchasascorbicacidAsAandglutathionereducedGSHinchloroplastofcv.Yuanyewerelowerthanthoseofcv.HelanNo.3, whileperoxidasePODactivityinchloroplastofcv.Yuanyewashigherthanthatofcv.HelanNo.3;ThesupplementofmethylviologenMV, thephoto-oxidant, enhancedtheproductionrateofO-·2, thecontentofH2O2 andMDAinchloroplastoftwospinachcultivars, whiletheadditionofAsA, theeliminatorforROS, decreasedROSlevelandaleviatedoxidizationofplasmolemma.ThecontentofchlorophylbChlb, chlorophylaChlaandprecursorofChlsuchasprotochlorophylPchl, Mg-protoporphyrinⅨ Mg-ProtoIX, protoporphyrinⅨ ProtoⅨ anduroorphyrinogenⅢ UroⅢwasremarkablydecreased, butthecontentofporphobilinogenPBGandδ-aminolevulinicacidALAwasincreasedbythestressofseawater, whichleadtoinhibitionforChlsynthesis, andtheinhibitionwasaggravatedbyMVandeliminatedbyAsA.ChlorophylaseChlaseactivityintheleavesofcv.YuanyewasimprovedwhileitwasnotinfluencedintheleavesofHelanNo.3underseawaterstress.TheChlaseactivityintheleavesofcv.YuanyewasmoregreatlyafectedbyMVtreatmentthanthatofHelanNo.3, however, ChlaseactivityoftwocultivarswasnotinfluencedbyAsA.TheseresultssuggestthatROSiscloselyrelatedtoChlmetabolism, whichnotonlyinjureplasmolemmabutalsoinhibittheprocesoftransationofPBGtoUroⅢ asaresultofChldecomposition.Inseawatertolerantcultivarcv.HelanNo.3, theROSeliminationismainlydependedonSODandAsA-GSHsystem, whichcouldaleviateoxidationinjuryofROStochloroplastmembraneandinhibitionofChlsynthesis, andChlaseactivitywaslessinfluencedbyseawaterstress;whileinseawatersensitivecultivarcv.Yuanye, itismainlydependedonSODandPOD, whichhaslimitedabilitytoeliminateROSandleadtoaccumulatemassROStosevereoxidationinjuryofchloroplastmembraneandinhibitionofChlsynthesis, andChlaseactivitywassignificantlyimprovedbyseawaterstress, whichenhancedChldecomposition.KeyWordsseawater;spinachSpinaciaoleranceaL.;chloroplast;reactiveoxygenspecies;chlorophyl s ÍChl ¹ o K1¥; ä Í,Ç V[ lv ; ,7 OÎî;“d;“dQÏ[ 1] 。±þ8 Chlc ¥Ú® | ¿Chlîг¥î ÿ Ü[ 2] 。Chl 3þî[3]¥ © BçCp¸,ûöYChl¥î,V7 Chlc ®;Chl³ ½\³;ij ÕQ Ë, Ï s Í ½ChlaseÐChl³¥1“ á M[ 4] ;ROS9 V Chl³[ 5],Yuzo©[ 6] ª¹ROS V Ð Chl4- 6Ì¥ åÊ,V7F ÎChl³。±þ®Ñ[ 7] 、SO2[8] 、NO2[ 9]、 £s} ç[5] 、 º[10]©Hq/,Chl³ÐROS}µ1;÷} ç/,ROS ÐChl³E]Chlî[E]ÊÄû ü 。 s8 Á 3ROS¥1 [11],÷} ç/ s8 ROSv Æ[ 12, 13] , s8 3VÄQ,; ä ͳ,Y ±þ;T¨。Z £} ç/,kSpinaciaoleranceaL. 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