不同种系梅花品种组织培养繁殖研究
p30 3 2008 M5 nbsp; “gt; “, y F “1Y。2.2 9 2.2.1 梅花品种的初培养 [,| V58 M/ ,M } 1 2MS 4, nbsp; k5 , Z、 [ M g F、 Q} 1。V1 3 } f 。V1 3 } f TABLE 1 Thepreliminary culture situation ofthree Prunusmume cultivars f V 3 FM 39; U 39; 3 f 39;,C2.2.2 增殖培养基的选择2.2.2.1 M 39;939; 4C,M 39;9 V, v Y k5 [, QL 3 f Kz 3 v、 、 ,7MS5 3 f K , 7 O k5 9“ ,7M2 V3 QLM1 {, 9“ M ,i O k5 “Bj ,yN9753 g ]“ F NK X nV2。V2 M 39;939; T1 TABLE 2 The comparison of culture results amongbasicproliferation media of Tie Guhong39; mm 3 mm9“ QL 4.17a 9.23a 36.88a 3.23aMS 1.90c 11.95a 21.52b 1.78cWPM 2.31bc 9.75a 20.17b 2.45bcM1 2.90b 9.03a 24.27b 2.83bM2 3.72a 10.88a 37.67a 2.90bM3 2.40bc 8.99a 20.71b 1.97c]B ]3 V UP 0.05 sA。V3、5、6、8、9]N。2.2.2.2 39; 、 39;939; VV3 V[ A, 39; 、 39;1KD9 sY WPM[MS , 3 f z,9“ Xr3[ , 8 39;1 p。 39; 7,MS 9“ d,i O 9 nbsp;; 39; s EsA,MS 3 {WPM , 3 V[,ZL nMS 。V3 39; 、 39;939; TABLE 3 Selection of optimal media on proliferationforMeiren39; andYanxing39; k5 cm 3 cm9“ 39; MS 2.34b 2.63b 6.15b 1.48bWPM 3.25a 3.05a 10.22a 3.05a 39; MS 3.68a 4.25a 16.64a 3.45aWPM 3.36a 4.05a 13.61a 3.32a2.2.3 铁骨红39;增殖培养过程中黄化问题的解决39;F V k5 Bn5,Y Z M i[ y M7 。N, 8 F 3 Y,7Y Y 3s, H 。AgNO3AgYV Ys8,V7 V ]YT。39;] H MY]4b ] i k5 CrT。V4 Vn,i1 MMS ,3 ib Hq/, k5 ,7 i2 3 MMS ,] ib Hq/, k5 , b 2 ,39;AgNO3 i4mg L H k5 CK A。8[ T , k5 1y M,9FM i,v ;Y 1y, b iVVQV YM T。V4 ] iMAgNO3 k5 3YTABLE 4 Effects of different concentrations of AgNO3 andFeon Mei flower culture in vitroMAgNO3 i mgL-1k5 f Ⅰ Ⅱ Ⅲ nbsp;x1 11MS 12 1.7 1.6 1.8 1.72 1 24 2.2 2.3 2.4 2.33 1 38 1.8 1.7 1.6 1.74 22MS 1 3.8 3.7 3.9 3.85 2 2 4.3 4.1 4.5 4.36 2 3 3.6 3.7 3.4 3.577 33MS 1 4.2 4.1 4.2 4.178 3 2 4.5 4.4 4.4 4.439 3 3 3.8 3.6 3.5 3.63K1 5.70 9.67K2 11.67 11.03K3 12.23 8.90k1 1.900 3.057k2 3.723 3.677k3 4.077 2.967R 2.177 0.710 IS k5 3 f s339;z、、,sY5 s 3 、4 C, 3、3, 3C、2C,C、1, 。2.2.4 碳源对铁骨红39;茎顶端死亡的影响]1 k, P[T k5 C AC。7 [ T ,5C z e。yN/B k P , I n39;y , P TM 39; 8 。8 ,M 39;KD9 339; QL ,39;QLv P MSs2MSM30 g L 。 F 、F1 5。M 39;9 nm1。2.2.5 增殖培养基最佳激素配比的筛选2.2.5.1 M 39;s QL 39; F3s 6--BA、KT、ZT, s | i, 3NAA, i0.1 mg L。40~ 50 d4 M 39;9rT,TnV5。F s 6-BA 、KTZT, 9]rT。VV5 V[ A,3s M 39; k5 8rT6--BAZTKT,7 O9“ 9sA, 6--BAZT i0.5 mg L H,9“ 76 nbsp; “gt; “。39;y 3 8 yN8“, “ M 39;F V、 45。M 39;KD F9 QL 1.0 mg L 6-BA 0.05 mg LNAA0.5 mg LGA3 , 3 1 2 QL0.3mg L NAA 0.1 mg L IBA; 39; KD F9 WPM 1.0 mg L 6-BA 0.1 mg LNAA, 3 1 2WPM 0.5 mg L NAA ; 39;KD F9 MS0.5 mg L 6--BA0.05mg LIBA, 3 1 2MS0.5mg LIBA。 ID[ 1] ..S m[ M] .S lt;,1989.CHEN J Y.Mei flower cultivars of China[ M] .BeijingChinaForestry PublishingHouse, 1989.[ 2], g . F CZ[C] f U.S4 \Z.S lt;,2004163--171.CAOL, L YM.Present state and progress of tissue culture inPrunus spp.[ C] nbsp;ZHANG Q X. Advances in ornamentalhorticulture of China.BeijingChina Forestry Publishing House,2004163--171.[ 3] HARADA H, MURAI Y.Micropropagation of Prunus mume[ J] .Plant Cell, Tissue and Organ Culture,1996, 364265-267.[ 4] OLAYA P T, LORENZO B.Different media requirements icropropagation of apricot cultivars[ J] .Plant Cell, Tissue andOrgan Culture, 2000, 632133--141.[ 5] QUOIRINM, LEPOIVRE P.Improved mediafor in vitro culture ofPrunus spp.[ J] .Acta Horti, 1977, 786437--442.[ 6] g , .. L.Z[ C] nbsp;f U.S4 \Z.S lt;,2004143--148.L Y M, CHEN J Y.Genetic breeding of Mei flower Prunusmume[ C] ZHANG Q X.Advances in ornamental horticulture ofChina.BeijingChina Forestry Publishing House, 2004143--148.责任编辑 董晓燕793 g ]“ F N/p