CO2加富对黄瓜叶片显微和亚显微结构的影响

p \   2002, 29130～34ActaHorticulturaeSinical 2001-05-24; 2001-10-09[ “  8O39;[ “J99I04CO2F A„A•Y魏 珉 邢禹贤 王秀峰 马 红  jlt;v\, 271018K 1[ k‹,CO2F A„A•Y。TV CO2F E F、V „ 9F,E F] 91  6,E M, † Fœ nbsp; ;  s8 ” “„vl、x ” “„vl、    ” 9F,† s8cx ”  6,Œ s8n A ]。 [‚] iCO2F3hr‹ l, 、/[1100100μLL-1CO2F3 hrTA。1oM;CO2F; ms |S 642.2 nbsp;DS M ’A nbsp;cI|0513-353X 200201-0030-05CO2F4T;† q] H,  iCO2 3 a‹ŸM。V,4CO2 i„ ’〔1〕、v〔2,3〕 9F,Œ ‚]。VuCCO2Fv,  s8x ”„89F, s8 A•Y〔3〕。P, CO29Hq/0„, x vl、 ” “ A9F,i•Y s8 vl、sƒ„ ”〔4〕。N Vn,‚]T „ 、‚] kHq/ CO2 a‹Ÿ。39; k[  k‹,CO2F A„AM, ] „9 。1 ‹ ZEk1999 M10～ 12  jlt;v\ k_„n39;†›。107 l,n 10cm ,rr„g3∶1
†,c1.45, rN 101.41mgkg-1, rP302.49mgkg-1, rK246.00mgkg-1。0Z 1016 sYM 4 nbsp;2.0m,z1.6m,1.0m  。 Ⅰ ,‚Fv; Ⅱ, F, CO2 i700100 μLL-1; Ⅲ, F, CO2 i1100100 μLL-1; Ⅳ, 、/ F, CO2 i1100100μLL-1。  60～80,g A8CO2,GXH-305˜“LCO2sNS iM。CO2F HW 800 830～1100 1130,/130 200～430 500。1116 | nbsp;Mj  i  。122  4˜3,VU/ ”3  M |l vsYFAA „。 k W4 nbsp;B5 „;HqM]。1016 1116  nbsp; K/K 20～ 35℃/8～16℃,11 H ;v lt;616.2 μmolm-2s-1,/2H ;v lt;465.4 μmolm-2s-1;1117 122 K/K 24～ 30℃/12～ 17℃,11 H ;v lt;306.3μmolm-2s-1,/2 H ;v lt;219.2μmolm-2s-1。1999 M2～3n39;†j  i› k,  I～ III,T39;]39;Q k。A4
 †   M |4～5mm2～3mmH,5FAA i, 3 „ A,TGMAM 。 8„ 3 „ A6～12h※• lt; b2Q Q3h※0‚ ※GMA 1A 2Q※GMA ˜※30℃ nbsp;†※LX-75˜ M  M※ ™ ※Ÿ Ž‹ 。OlympusAH-IIA 39;//4vM。  420～30 j7,9 。A4 †   M |vl1～ 2mmnZl v,4 „ -, pH7.2 A , 1 ,0Y-d‚ , Epon-812 Ž ˜, LKB-5˜M  M ,Z -  ™ , JEM-1200EX˜i 4、 ivM。  420～ 30 j7, | 。2 Ts2.1 nbsp;A2.1.1 nbsp;叶片厚度 CO2F„  9FV1。vM1, CO2700100 μLL-1„CO21100100μLL-1F  sY9F0.64„3.46, †1100100 μLL-1CO2F 9F12.77, -€vrAs,€As,  [‚] iCO2F3hM1, †[CO21100100μLL-1F6hrT A。  |  „V ,7 F“ E F“Z F。V1 A, CO2F E F„V ] H9F,E F 91yCO2F{  6。Z F‚]F iWs‚A。V1 nbsp;CO2F  MTable 1 Changes in leaf thickness of cucumber with CO2 enrichmentCO2 iCO2 concentrationμLL-1HWTime Leaf thicknessμmV Epidermisthickness μmE FPalisadetissuethickness μmZ FSpongy tissuethickness μmE F/ PT/LTvControl 150.58bB 23.24cB 44.65 cB 83.90bA 0.30700100 AM 151.54bB 25.54bAB 47.31 bB 80.13bA 0.311 100100 AM 155.78bB 25.73bAB 47.36 bB 82.92bA 0.311 100100 /AMPM 169.54aA 26.84aA 54.56 aA 88.35aA 0.322.1.2 nbsp;细胞大小 V2V , CO2F P E F9F,Œ 700100 μLL-1„1100100 μLL-1 sY9F5.5„5.7,vs‚A; †CO2F€9F26.6,sA。E F z‚] WAs。E F/1Z „ z9C M˜。CO2F P V  z9F,‚]F iWV M‚ A。 †1100100μLL-1CO2F P  „V ˜ M  ”Z A,89v。V2 nbsp;CO2F vl•YTable2 Effects of CO2 enrichment on the size of leaf cells in cucumber μmCO2 iCO2 concentrationμLL-1HWTimeE Palisade cellLengthzWidthZ Spongy cellLengthzWidthV UpperepidermiscellLengthzWidth/V LowerepidermiscellLengthzWidthvControl 43.76bB 12.61aA 24.44bB 18.54aA 21.61 bB 13.53bB 15.93bAB 9.83bB700100 AM 46.16bB 13.09aA 24.45bB 19.05aA 21.23 bB 14.93 aAB 15.69bA 10.73abAB1 100100 AM 46.26bB 12.86aA 25.97bB 18.85aA 21.72 bB 15.03 aAB 15.82bAB 10.99aAB1 100100 /AMPM 55.39 aA 13.09aA 29.92aA 19.14aA 24.30 aA 15.43 aA 17.14aA 11.64aA ˜ M ,
V ›Z_,d9125μmS E „1Z  ” “,Tnm1。1100100 μLL-1CO2F P  ” “A9F,ƒ CO2F‚ PE  †Fœ nbsp;、 ,7 O\Z  † _Mm, A～D。2.2 nbsp;A2.2.1 nbsp;叶绿体和淀粉粒 V3 V, CO2FHq/,E   s8 ” “9F,89v。311 nbsp; nbsp; nbsp; nbsp; nbsp;  CO2F A„A•Y nbsp; nbsp; nbsp; nbsp; nbsp; 700100μLL-1„1100100μLL-1CO2F P c s8 ”sY1v9F16.90„27.46, †F9F25.64; [‚] iCO2F€ s889F l,†F€9FA, „ zsY1v9F8.36„26.49。[ T CO2F\ s8™„。CO2F P E  x ” “9,89vV3,m, E～H, †i  †。v c9.14x ,  s8‚ž1,O8 l; 700100μLL-1„1100100 μLL-1CO2F P  x ”sY9Fž16.22„23.72,  s8c1～ 2; †CO2F  x ” “B„9F,  s8c2～ 3, O8A9v。m1 CO2F E 、Z  ” “•Y125μm Fig.1 Effects of CO2 enrichment on the nubmer of palisadeand spongy cells in125 μm length of cucumber leavesV3 CO2F   s8、x ” “vlMTable3 Number and size of chloroplast and starch grainin leaves of cucumber with CO2 enrichmentCO2 iCO2 concentrationμLL-1HWTime s8 ”/ Chloroplast number/cell s8ChloroplastLength μmzWidth μmx ”/ Starchgrain number/cellx Starch grainLength μmzWidth μmvControl 10.92 cB 5.61 bA 1.85bA 9.14dB 0.59 cC 0.23 cC700100 AM 12.77bA 6.17 abA 1.94bA 16.22 cB 1.70 bB 0.88bB1 100100 AM 13.92 aA 5.86 abA 1.90bA 23.72 bA 1.86 bB 0.87bB1 100100 /AMPM 13.72 aA 6.64 aA 2.34aA 29.00 aA 2.76 aA 1.54aA2.2.2 nbsp;基粒和片层 CO2F‚•Y s8vl„ ” “,7 O•Y 。F   s8 ” “9FrAs ;  ”9F, AM, 58,c  9V4。   CO2F i9F„ HW7†,Œƒ  9F W9v,。V4 CO2F s8   •YTable 4 Effects of CO2enrichment on grana and lamellae of chloroplast incucumberCO2 iCO2 concentration μLL-1HWTime ”/ s8Grananumber/chloroplast Granathicknessμm ”/ Lamellanumber/grana Lamellanumber/μmvControl nbsp;15.55aA 0.135bB 10.39bB 76.96cC700100 AM nbsp;15.64aA 0.163aAB 12.06abAB 73.96bB1 100100 AM nbsp;16.36aA 0.183aA 13.22aA 72.25aA1 100100 /AMPM nbsp;17.18aA 0.173aA 12.33aAB 71.29aA3  ‚CO2F„  9F,ƒ’、vTM],Œ9Fy‚]。Radoglou kV ,’ 9F1 ,amp;shy;  Z  CFM ,E FZ F1i‚M, 8Mv,amp;shy; ˜ M  ”Z A,7E  ”、V  、  d „  d ”‚ s•Y〔1〕。v 9FE  z„ ”“9F〔3〕。CO29 3v, /V amp;shy;v ’V ˜ š, y9F32 nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp;  nbsp;\ nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp;29 BE F7 AM,  d /†〔2〕。39; k, CO2F 95  „V ] H9FT,E  ”‚MŒ A ,E F] 1  6, † Fœ nbsp; ,E FMZ F † 9 t。N A,‚]T 4CO2 i a‹Ÿ‚],s „ ”f V „ My。4CO2 i‚]T  s8•Y9‚B“。CO2F   s8、x ” “9F,89v, ”„  ”9F,Œ  s8 cx ”“ V3,•Y s8ž。CO2FI N x vl、 ” “9F, 58M™9K †  ” s8〔5〕。Œ , CO29Hq/0„,x vl、 ” “ 9F, Ox 8„ ” •Y s8 vl、sƒ„  ”〔4〕。 nbsp;39;T, CO2F\ „ s8。 - V , ;† q | „E F〔6〕;v  s8  ”;† qžM1〔7〕。 i“};  “,] HT  † O, ™B}†. {,\;  l.„。yN, CO2FHq/ A„AM V  iCO2 a‹,[4; „CO2 nbsp;l  ; 6BZ ,CO2 i/ ;† q4,/„  †,  。NB„。 – [‚]iCO2F  3 B•Y,Œ†sSviAs,7 †CO2F M A,ƒ CO2F HWTv。• ID1 RadoglouK M, JarvisPG.Theeffectsof CO2 enrichmentandnutrient supplyongrowthmorphologyandanatomyof Phaseolusvulgaris L.seedlings.Ann.Bot., 1992, 70245～ 2562 nbsp;’,.v CO2 i 6Q‹., 1996, 38131～ 343 VuJCV, AllenLHJr, BowesG.Leafultrastructure, carbohydratesandproteinof soybeansgrownunderCO2enrichment.Environ.Exp.Bot.,1989, 29 2141～ 1474 P,{I , X,.CO2 i90„, s8r‹., 1996, 38 172～ 765 YelleS, BeesonR CJr, TrudelM J, et al.Acclimationof twotomatospeciestohighatmosphericCO2.PlantPhysiol., 1989, 901465～ 14726 Delayney RH.Morphological and anatomicalfeatures of alfalfa leaves relatedtoCO2exchange.CropSci., 1974, 14444～4477  o , [ j.v‚] 3 3 ‚]3;†Ÿs 1“.T, 1992, 18 3191～ 195Effects of CO2 Enrichment on the Microstructure and Ultrastructure of Leavesin CucumberWeiMin, Xing Yuxian, WangXiufeng, andMaHongCollege of Horticulture, Shandong AgriculturalUniversity, Tai39; an 271018AbstractThemicrostructureandultrastructureofleavesincucumberwithCO2enrichmentwereinvestigated.It showedthatthethicknessofpalisadetissue, epidermisandleafincreasedsimultaneouslyinCO2-enrichedleaves,andtheratioofpalisadetissue to leaf thicknesswent upslightly.Longerandmoretightly arrangedpalisade cellswereobservedinleaves withCO2 enrichment.Not only the numberandsizeof chloroplast andstarchgrain, butalsothenumberofgranaandlamellaincreasedasa resultofCO2enrichment.Noobvious chloroplastdeteriorationandthylakoiddeationwerefoundinCO2-enrichedleaves.Enrichment at differentCO2 concentrationsforthreehoursinmorningwasnotvery effective, but enrichment forsix hours at1100100 μLL-1 everyday producedmost significanteffects.Key wordsCucumber;CO2enrichment;Leafstructrue331 nbsp; nbsp; nbsp; nbsp; nbsp;  CO2F A„A•Y nbsp; nbsp; nbsp; nbsp; nbsp; m A～D A150;E～ H 10000。A、 E,v;B、 F, CO2 700100μLL-1, F;C、 G, CO2 1100100μLL-1, F;D、H, CO2 1100100μLL-1, /F。Explanationof plate A-D leaf microstructure 150;E-H leaf ultrastructure 10000.A, E, CK;B, F, CO2 700100μLL-1AM;C, G, CO2 1100100μLL-1 AM;D, H, CO2 1 100100μLL-1 AMPM.34 nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp;  nbsp;\ nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp; nbsp;29 /p