亚精胺对黄瓜幼苗灰霉病的诱抗作用
h FT*尚庆茂** 张志刚S jY20[ , H Vr50[ 11] . h 7W,“ S , VI N、 N0、 X、、7、、 T、1 3 2008 M4 19 4 ChineseJournalofAppliedEcology, Apr.2008, 194825-830DOI 10.13287/j.1001-9332.2008.0211200、T L s F[ 12] . “ -, h[ j0], j0 PQ 、i9F,h F9 ,]rT9 , j0 ,] rB. k[CucumissativusL. ,Spd h S / h f 、] FhM1c Y,[ Spd F hrT V 3 , F4 hp4 LlG .1 ZE1.1 k 4|.SpdSIGMA } S2626, A8, 0.925 gL-1,B≥98, |Spd2.206 ml, 1 000ml 2 000 mgL-1 A,. h S/ S 3 i4.k2005 MS j S v j i .04h,b24h,315 4 B0l n9 cm9 cm, F[ 13] .iHq233℃/122℃,1 ;v ; , M A75~95.1.2 k ,Spd 0v、50、100、150、200、250 mgL-1“ i. uF 9, 3Q, l u20.46 2Z H, |Spd 2 cm S, 20 ml, HSpd A 1n;2d , l u 60ml, 、 A /.v }9.1.3 h 410 , S4d h .sY f0A ZE.fu d u |0.4cm f2 , P 1 f.h,9 h f .0A 0A i4.3105ml-1. 9 |0A,|2 0A5 s, TV]0A . S 3 3S.f0A] H.85{ 5 ,212℃/151℃, b A90[ .1.4 ZE1.4.1h f sY2、4、6 d E3Eh、, Th,h f 9 T /h f ∑ h h Kvh1001.4.2 3 3S sY3、6、9、1215 dg | 2 3 3S.vMoerschbacher[ 14]ZEPAL、POD;vMiler[15]ZEPPO;vo[ 16]ZE9p、 [c ;vMorison[ 17]ZE c .1.4.3 S 426 , S20d, 、Y、 、 、 7 . Y/ 100. SAS qDuncanE1 sAs,mV 3Q .2 Ts2.1 Spd h f Ym1 V,vM1,Spd A m1 Spd h f YFig.1 Effectsofexogenousspermidineondiseaseindexincu-cumberseedlings.Ⅰ0mgL-1Spd;Ⅱ50mgL-1 Spd;Ⅲ100 mgL-1 Spd;Ⅳ150mgL-1 Spd;Ⅴ200mgL-1 Spd;Ⅵ250 mgL-1Spd.826 3 19 h f .] H 2、4、6d,Spd ih f CM]M p, Spd iv 6,h f v,B, “Spd i 679F. Spd h f i aR i,[150mgL-1 K,2、4、6 dh f sY1v 10.0、18.525.4.2.2 Spd h S / ] YV1 V, S HW, S6、9 d,POD Spd i 65 6M t ]; S15 d, Spd i 6,POD5 6M t ].] H ,Spd i PPOv,SpdPPO9 9VCB ir. S3、6 d,Spd i150mgL-1 H, PPO Spd i 679 ;Spd i150 mgL-1 H,PPO Spd i 67h ;Spd i150 mgL-1 H,PPOrKv,sY1v4 15.527.5. S9、12、15 d,PPOC200mgL-1 ,sY1v9F 38.9、22.629.7.2.3 Spd h S / FhM1c YV2 V, S HW,9pc 5 6 M t ]. h - P3 d,Spdi 9pc v,9pc Spdi 679F,Spd i200mgL-1 HrKv,1v9F13.7; h P6、9、12、15 d,Spd i 9pc v 6,v.V1 Spd h S / ] YTab.1 EffectsofexogenousspermidineonactivitiesofdefenseenzymesincucumberseedlingsleavesunderinfectionofB.cinereaSpd iSpermdineconcentrationmgL-1 S9 d,Spd i [c vAs; S12、15 d,Spd i [c v.] H ,Spd i c v,Spd \TVCB ir. S3、6、9 d,Spd i150 mgL-1 H, c Spd i 679F,Spd i150 mgL-1 H, c Spd i 67h ,Spd i150 mgL-1 H, c rKv,sY1v4 17.8、52.023.6; S12、15 d, c C200 mgL-1 ,sY1v9F18.344.5.2.4 Spd h S / 3YV3 V,Spd h S / Y、、9 、 7 、 、 6[S A\T;Z 7 9\T,vM1sA.Spd 3\TVC A ir, iv 6,Spd \Tv9 ,[SrKv, Spd i 6,\Tvh .100 mgL-1Spd 3\TKv,Y、9 、 、 sY1v4 8.3、35.2、49.432.5.3 “ -,1Spd Fh[\ 37n.b[ 18]V ,d IHq/ Spd Vr rT. kTV ,Spd V h F,h f 1vK25.4;\ h S / 3,vM1, K9F32.5.PAL、POD、PPO Fh}V1o. FhQ“ Es M1“,YT 8 ]Q1S.PAL 6BZ , X] s0,Y ,V7 .TV ,Spd PAL、POD、PPOC]M t ], S HW,PALv,PODv9 ,PPO5 6M t ].M t ]v HW B,Spd 3 k WBv ,PAL、POD、PPO3] KsY1v9F48.4、635.938.9.yN,t Spd F hBM1, V YV4828 3 19 t 9 h S 、 .p } . Fhy0, “h S T, FhB[ 22].9p [ p , }PAL、POD、PPO MM1. C1sB,h 3 S] pT,7 O s0 p -8[ T3 V[ 、 [23] . kTV , h - P3 d,Spd\ 9p、 [}; h , - P6 dSpd\ [},7] 9p}, P12、15 dSpd\ 9p},7] [}. k W,Spd\ }. Spd V YV8 9p、 [、 } 4 h F.N, k] FhM1c M t ]VCiB, FhM1c C HW is, V Spd F|. HW s , s0 B.B ,T P I} |Q[ 24-25] .V , PAOs H 3H2O2 V Cvs0 Fh1, QHR“d FSAR|.V[ 26].Shen[ 27]C} /,Spd VE 8NADPH 4,V7] } H2O2c 6,4 0 . 6, SA 6 YVSpm[ 28]. sC.gloeosporioiddes , V[Ah“ s 30,i] .]r1 ] Ca2/CaMG |.V[ 29].Kasukabe[ 30]C, Spd cDNA E 2 ,Spd Spdc v A9F,] H9 、、、l、 1i} j]} F.cDNA sV , I} /,yBtyv c, } Y cy0 DREB} rd29A.V Spd VT} |. {o|y0,y} F. 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