浙江省西(甜)瓜蔓枯病菌对甲基硫菌灵和啶酰菌胺的抗性检测及抗性机制.pdf

研究论文 doi 10 16801 j issn 1008 7303 2021 0129 浙江省西 甜 瓜蔓枯病菌对甲基硫菌灵和 啶酰菌胺的抗性检测及抗性机制 吴倩倩 庄超杰 吴鉴艳 张传清 浙江农林大学 现代农学院 杭州 311300 摘 要 从浙江省5地采集了112株西 甜 瓜蔓枯病菌Stagonosporppsis citrulli 采用区分剂量 法检测其对苯并咪唑类杀菌剂甲基硫菌灵 以下简称Ben 和琥珀酸脱氢酶抑制剂类 SDHIs 杀 菌剂啶酰菌胺 简称Bos 的抗性 结果显示 112株西 甜 瓜蔓枯病菌对Ben和Bos的抗药性 频率分别为100 和28 6 其中对甲基硫菌灵产生高水平抗性 BenHR 的菌株达100 对啶 酰菌胺产生低水平抗性 BosLR 和高水平抗性 BosHR 的菌株分别为18 8 和9 8 抗药性分子 机制研究表明 BenHR菌株中 tubulin的第198位氨基酸由Glu E 突变成了Ala A BosHR菌 株中Sdh B的第277位氨基酸由His H 突变成了Tyr Y 但BosLR的抗性机制还需进一步研 究 研究结果表明 浙江省西 甜 瓜蔓枯病菌对苯并咪唑类杀菌剂甲基硫菌灵的抗性已十分严 重 尽管大多数菌株对啶酰菌胺仍表现敏感 但在一些地区已存在高水平抗性菌株 应在加强 抗药性监测的同时 注意SDHIs类杀菌剂的科学使用 关键词 西瓜 甜瓜 蔓枯病菌 甲基硫菌灵 啶酰菌胺 抗药性检测 抗药性机制 中图分类号 S481 4 TQ450 13 文献标志码 A 文章编号 1008 7303 2021 06 1117 06 Resistance monitoring and mechanism of Stagonosporppsis citrulli to thiophanate methyl and boscalid in Zhejiang Province WU Qianqian ZHUANG Chaojie WU Jianyan ZHANG Chuanqing College of Advanced Agriculture Zhejiang Agriculture Forestry University Hangzhou 311300 China Abstract A total of 112 isolates of Stagonosporppsis citrulli were collected from 5 locations in Zhejiang Province The resistance to thiophanate methyl Ben and boscalid Bos was detected by the method of differentiated doses The results showed that the respective resistance frequencies were 100 and 28 6 to Ben and Bos respectively The high level resistance BenHR of thiophanate methyl reached 100 and the low level resistance BosLR and high level resistance BosHR of boscalid were 18 8 and 9 8 respectively Further studies on the molecular mechanism of resistance showed that the BenHR of S citrulli was caused by the E198A point mutation in tubulin which was the mutation of 198th amino acid of from Glu E to Ala A The mechanism of BosHR was the point mutation in 277th amino acid of Sdh B from His H to Tyr Y but the mechanism of low level resistance BosLR still 农药学学报 2021 23 6 1117 1122 Chinese Journal of Pesticide Science 收稿日期 2021 04 28 录用日期 2021 08 17 作者简介 吴倩倩 女 硕士研究生 E mail 1357791079 张传清 通信作者 Author for correspondence 男 博士 教授 主要从事杀菌剂生物学与植物病害治理研究 E mail cqzhang9603